Primer

Part:BBa_K5427069

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


RV_KerDZ_pET22

Reverse primer for amplification of KerDZ insert in pET22b+.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 40
    Illegal PstI site found at 26
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 40
    Illegal PstI site found at 26
    Illegal NotI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 40
    Illegal PstI site found at 26
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 40
    Illegal PstI site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None